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395,396 Synthetic vesicles in PURE have also been used to systematically stop and start translation to study membrane protein folding and the effects of different lipid compositions
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A mixture of degenerate forward primers (Deg1–Deg7, Supplementary Table 3) was designed from κ-chain sequences in the Kabat database 52 and used with the 3′ reverse primer LC-9 (Supplementary Table 3) to amplify the complementary DNA (cDNA) sequence of the IgG κ-LC gene
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